Darn Test

Jialat...

So tired again... Strech.. yawnz...
Did the stupid test... so far all was well, but in one part the selection of certian colonies of cells chosen wrong. WTF?
My criteria of choice was 30 - 300 CFU, while in that, any amount of CFUs also can be used, i forgotten that total CFU undiluted under 300 can liow... sheez. Basics all fogotten.
Anyway, went to arcade after work for a bit.
Seen DM, GF, DDR... got players, feel sian to wait so decided to go home.

NO pics today too :P How to smuggle pics of the exams?? whahahaa...

Anyway, my thoughts... should be more life to bemani games. I guess I can pursue my interest in writing music again, playing the keyboard, skating, losing weight?? lol... NO.
Really should concentrate on studies...
HPP, MGMB, CABL... all 3 killer subjects.

I guess I will have the highest confidence in CABL, being most interested in Biochem the most. Followed by a large distance, MGMB and then tightly follows HPP.
HPP is really a strange subject. We have to study the human physiological aspect, as well as pharmacology aspect. How much are we suppose to know, or how much do we need to write?
Well I guess this is suppose to be 2nd nature to uni students. Have to be mature to know as much as possible and to attempt the impossible.

Lets see... I covered DNA repair mechanisms today.
DNA damanged (Potentially at 260nm) because closer to frequency of UV light. Damaged DNA will form 2 type if dimers, one potentially lethal (cyclobutane dimer) and one is highly mutagenic (6-4 photoproduct).
Resolution of damaged DNA comes in 2 forms of mechanisms.
Light dependent and light independent mechanisms.

Light dependent (requires photo activation)requires an enzyme as photolyase, it binds to pyrimidine dimers and uses energy from visible light specturm (370nm) to split the dimers apart.

Light independent (3 types)
* Excision repair - removal of damaged DNA strand followed by synthesis
* Recombinational repair - uses the good 3' 5' stran for repair
* SOS error pronerepair - simply put, anyhow dump nucleotides to try salvage cell from death.

Excision repair - uvrA and B ID dimer damages, A then leaves and C binds to B and introduce nicks on either side of dimer (8 nucleotides on 5' and 4/5 on 3' side)
After which urvD removes damaged strand, and repaired by DNA polymerase I and DNA ligase.

Recom repair - RecA protein is important in this repair. Undamaged parental strand recombines into gap opposite dimer leaving gap on the other parental strand. Gap in the undamaged parental stand filled by the DNA pol I and ligase, thymine dimer can now be repaired by excision repair (refer to above).

Haven cover SIS error prone repairs... very tiring lol...
But i know recA and lexA regulates it ba... after which 30 protein genes are expressed bleh... too many to learn now. Gonna log off and sleep.


Prayer for my love...

My love my love,
to God I pray,
every once in the night,
when the stars shine bright.

My love my love,
When I pray, I say,
To you everyday,
thy heart won't be grey.

With true love I say,
I will be with you always,
Thru all hardships will endure,
I embrace you in the end of fall.

Winter comes, my heart won't go,
No void promises, true as gold,
like rose petals, delicate soul,
my soft hands to hold your soul.


My dear, this I give you. Though its all words...
Please forgive me for I have been busy.
I don't have time to provide you happiness.
Please don't be angry, nor forget me.

In essence, I give my heart to you and to say I like you a lot.


Ok... upcoming for me...
Thurs morning: R&C Brainstorm meeting
Thurs afternoon: Curatorial meeting
Monday: CABL Test

Have to remember to give Michy the MS office pro disc...
And DL the drumming game for her ^_^